Cell Culture Services

CELL CULTURE SERVICES

KTI offers a wide-range of cell culture services associated with the establishment, expansion, cryo-preservation and storage of normal and atypical cell lines. We provide a highly effective and efficient arrangement for your experimental needs of cultured cells. Cultures are established to meet your specifications for animal source, dishes for plating, cell density and any other specified parameters. You can have 'just-in-time' delivery of healthy and ready-to-use cultures on the day and time of your choice. Cultures are established with a few days notice for the first time and on specified delivery days thereafter. We take the worry in our hands of acquiring and handling animals for primary cultures and specific media required for each culture type, so you can focus more of your attention on experimental aspects. KTI technical staff works closely with the investigators to identify and comply with their specific needs. We are equipped and experienced to provide following cell culture related services.Primary embryonal cultures such as neuronal, fibroblasts, lymphocytes etc. , cell line cultures, cultures after various treatments are provides as test materials for various investigations.

Isolate Peripheral Blood Mononuclear Cells (PBMCs) from whole blood specimens.
Activate and establish short term primary cultures of B-cells, T-cells and Macrophages.
Establish short term Primary cultures of Neurons, Astrocytes and Fibroblasts.
Establish immortal B-cell lines by Epstein Bar Virus (EBV) transformation.
Develop, re-clone and grow hybridomas for generation and production of monoclonal antibodies.
Expand and grow cell lines to harvest large amounts of cells or secreted products including microbial agents.
Custom develop drug testing protocols including high throughput assays.
Grow cell stocks to client specifications for transfection studies, induction of differentiation of tumor cell lines, or plate them on multispot or multichamber slides for downstream assay systems. Cultures on slides could be fixed and used for educational purposes.
Cell based diagnostics including microbial infections using In-situ hybridization assays, Immunoassays on tissues and cells including immunefunction assays, cytotoxicity assays and HLA typing.
Cryopreserve and store PBMCs and cell stocks in duplicate liquid nitrogen vapor phase freezers at our biological repository facility.
Ship frozen or viable cultures worldwide.