ESTABLISH PRIMARY CELL CULTURES IMMORTALIZE & GROW B-CELLS EXPAND TUMOR CELL LINES CELL BASED ASSAYS
KTI offers a wide-range of cell culture services associated with the establishment, expansion, cryo-preservation and storage of normal and atypical cell lines. We provide a highly effective and efficient arrangement for your experimental needs of cultured cells. Cultures are established to meet your specifications for animal source, dishes for plating, cell density and any other specified parameters. Cultures are established with a few days notice for the first time and on specified delivery days thereafter. KTI technical staff works closely with the investigators to identify and comply with their specific needs. Following are the examples of such cell culture operations at KamTek Inc.
1. PRIMARY CELL CULTURES
---PRIMARY CULTURES OF HUMAN PBMCs
---ANIMAL PRIMARY CULTURES
2. ESTABLISH B-CELL LINES BY EBV TRANSFORMATION
Buffy coat isolated from human blood sample received from a donor is transformed with EBV, and in 4-8 weeks, continuously growing culture can be established as a resource of:·
KTI technical staff works closely with the investigators to identify and comply with their specific needs. For example, depending on the needs of the project, EBV transformation may be set up with:
Transformed cells are grown and cryo-preserved for future use. In addition, cell line growth can be continued to harvest cells for DNA isolation. Cell stocks are always tested for lack of mycoplasma contamination and can be validated for specific characterization before cryo-preservation. Alternatively, bulk cultures prepared can be shipped to client for testing. Cryo-storage services are provided at clients request.
All cultures expanded at KTI go through MYCOPLASMA TESTING at least at two stages-- at the mid point of expansion and when culture is fully expanded.
4. CELL BASED ASSAYS